Phytochemical analysis of Citrus limetta using High-Resolution Liquid Chromatography Mass Spectrometry (HR-LCMS) and FTIR

Abstract

Sweetlime peel (SLP) is a rich source of bioactive chemicals. The nutraceutical sector claims that a large range of bioactive secondary metabolites produced by sweet lime peel can be exploited to develop novel pharmaceuticals and functional foods. It has been proven that it is critical to analyze complicated bioactive substances employing analytical methods such as HR-LCMS. The current project's purpose was to use HR-LCMS and FTIR to investigate the bioactive metabolites contained in SLP methanolic extract. Investigating the bioactive components of SLP using Q-TOF LC/MS and Agilent MassHunter Profiler software revealed 18 flavonoid compounds along with polyphenols like rutin, 4',5,7-Trihydroxy-3-methoxyflavanone ,Curcumin diglucoside, carotenoid, Coumeroic acid, coumaric acids, flavonoid-7-o-glycosides, and Gardenin B that have
anticancer, antimicrobial, anti-inflammatory, and antitumor activity. The results of the present study confirm the presence of important phytocompounds in SLP and are useful for further in-depth research to create functional foods from SLP to prevent a variety of ailments. Current research aims to discover all-natural therapies for a variety of illnesses and conditions.