Influence of native microbial biocontrol agent on radial growth of Fusarium oxysporum f. sp. ciceri isolates

Abstract

Microbial biocontrol agents (MBCA) play an important role in managing various plant pathogenic diseases. Several bacteria and fungi are known to act as MBCA and one of the fungi, the Trichoderma spp. is considered as most effective MBCA against numerous fungal plant pathogens. Earlier reports revealed that it acts through different mechanisms and inhibit the plant pathogens. Fusarium oxysporum f.sp ciceri (FOC) is one of the soil born plant pathogen which can survive in soil for a longer period and it largely infect the highly nutritious chickpea pulse crop causing chickpea wilt disease. In present study, the effects of secondary metabolites released from the native MBCA i.e. T. harzianum was investigated by using series of experiment on seventeen representative FOC isolates of Bundelkhand region of India. Findings of present study indicated that the secondary metabolites released from native T. harzianum were significantly found effective against seventeen representative FOC
isolates. In Dual culture technique it significantly inhibits radial growth of MPFOC42 (65.19%). In addition secondary metabolites released in the form of volatile compounds at 2 DAI to 4 DAI show higher percent inhibition in MPFOC 37 (from Mudara village of Tikamgarh district) i.e. 45.45% at 2DAI and 35.29 % at 4DAI, and as well as through non-volatile secondary metabolites highest radial growth inhibition was observed in MPFOC21 (isolated from Sonagiri village, district Datia) i.e. 71.11, 78.52 and 83.70% at different concentration of culture filtrate i.e. 10%, 15%, 20% respectively. These findings indicate high efficiency of native MBCA i.e T. harzianum, which significantly inhibits radial growth of FOC isolates of Bundelkhand region.